Nonhistone chromosomal PROTEINS are a set of heterogenous chromatin PROTEINS which play different functions dependent on the cell type. A group of these PROTEINS with a high molecular weight and low mobility on the gel electrophoresis, called LMG PROTEINS, has been the subject of many workers. We have recently isolated a fraction of these PROTEINS from rat liver nuclei, with a molecular weight of 160 KDa and named HMG160.In this study the position of this purified protein in different fractions of chromatin has been studied. After brief digestion of rat liver chromatin with MNase, chromatin fractions S1 and S2 were prepared and analyzed by gel electrophoresis and imminobloting techniques. The results show that the protein LMG160 has higher affinity to di- and oligonucleosomes rather than to mononucleosomes. This implies that LMG160 preferentially binds to bulk chromatin and may have a role in chromatin inactivation which demands further investigation.

Aim: The aim of the present study was to compare the effectiveness of Emotion-Focused approach training based on HMT/LMG and transactional analysis on the differentiation and resolving the Parent-Adolescent conflict. Method: The research method was quasi-experimental with a pretest-posttest study of two experimental groups with a three-month follow-up and the statistical population included all female high school students in Mashhad in the academic year of 1399-1400 (12018 people). The sample size included 60 students along with their mothers who were selected by multi-stage cluster sampling method and randomly selected into two experimental groups (each group 30 people). In order to collect data from the measures of the Child’s Attitude toward Mother Scale (CAMS), Murray Strauss conflict tactics (1990) and Strauss and Skorn and Friedlander's differentiation (1998), as well as Stewart and Joines (2009) transactional analysis training packages and Aikin and Aikin's (2017) Emotion-Focused (HMT/LMG) were used. Both groups completed the mentioned questionnaires and then one group received the transactional analysis intervention and the other group received the HMT/LMG program. At the end of the intervention, both groups completed the questionnaires. Results: The effect of time, group and time interaction in the Emotion-Focused (HMT/LMG) training group was significant for differentiation and conflict resolution compared to the transactional analysis group (p>0. 001). Conclusion: According to the obtained results, program HMT/LMG is recommended in Emotion-Focused training to increase differentiation and resolve conflicts during adolescence.